1 plate of Vita-Assay™ coated with the plain CAM film (N), red fluorescent CAM (R) or green fluorescent CAM (G). 1 tube of Cell Releasing CAM Enzyme. *

102.01N - US $250 per kit; 102.02R - US $300 per kit; 102.03G - US $300 per kit

6 samples of 1-10 x 1,000,000 nucleated cells, i.e., nucleated cells in 1-mL of whole blood shall be loaded into one well of the 6-well plate. One Vita-Assay™ RCE AN6W plate can accommodate a total of 6-mL whole blood.

Recommended to use with the nucleated cells prepared by red blood cell lysis of whole blood.

Whole blood, bone marrow or nucleated cells.

Flow cytometry, immunocytochemical staining, FISH, etc; Quantitative RT-PCR, DNA microarray, SAGE, proteomics and genomics.

Vita-Assay™ AN6W series is for the functional enrichment of viable tumor progenitor and other rare stem cells from whole blood, lymph, bone marrow and other bodily fluids. Enriched cells can be analyzed by microscopy, flow cytometry and PCR. Enriched cells can also be cultured in the same device for functional analyses of viable tumor progenitor and other rare cells, including immuno-phenotyping, counting, genomic and expression profiling, as well as CTC drug sensitivity (CDS) testing.

* CAM-avid cells released from CAM scaffolds by Cell Releasing CAM Enzyme plus Trypsin/EDTA shall be concentrated to a small volume, i.e., 100 µL PBS containing the enriched cell fraction. The 100 µL cell suspension can be processed for cellular analyses such as fluorescence microscopy, FISH and flow cytometry.

6 tubes of Vita-Cap™ N9 coated with the plain CAM film (N9), red fluorescent CAM (R9) or green fluorescent CAM (G9). 1 tube of 10x anticoagulant blood diluents. 1 tube of Cell Releasing CAM Enzyme. *

104.01N - US $600 per kit; 104.02R - US $675 per kit; 104.03G - US $675 per kit

6 samples of 1-mL to 3-mL whole blood or bone marrow plus 4-mL of anticoagulant working medium.

Whole blood, bone marrow and peritoneal effusions.

Flow cytometry, Fluorescence microscopy, FISH, etc; Quantitative RT-PCR, DNA microarray, SAGE, proteomics and genomics.

The Vita-Cap™ N9 RCE kit is for the functional enrichment of viable tumor progenitor and other rare stem cells from whole blood, lymph, bone marrow and other bodily fluids. Enriched cells can be analyzed by flow cytometry and microscopy for immuno-phenotyping and counting of CTCs and CTPCs, as well as other molecular analyses, including proteomics, gene expression profiling, genomic and mutational analyses.

* CAM-avid cells released from CAM scaffolds by Cell Releasing CAM Enzyme plus Trypsin/EDTA shall be concentrated to a small volume, 100 µL PBS containing the enriched cell fraction. The 100 µL cell suspension can be processed for cellular analyses such as fluorescence microscopy, FISH and flow cytometry.

** CAM-avid cells released from CAM scaffolds by Cell Releasing CAM Enzyme plus Trypsin/EDTA shall be concentrated to a small volume, 10 µL PBS containing the enriched cell fraction. The 10 µL cell suspension can be lysed for protein, mRNA or DNA isolation, followed

6 chamber slides of Vita-Assay™ coated with the plain CAM film (N), red fluorescent CAM (R) or green fluorescent CAM (G); each chamber slide contains 16 individual wells. 1 tube of Cell Releasing CAM Enzyme. *

101.01N - US $750 per kit; 101.02R - US $810 per kit; 101.03G - US $810 per kit

6 samples of 1-10 x 1,000,000 nucleated cells. 1-10 x 1,000,000 nucleated cells in 1-mL of whole blood shall be divided and loaded into the 16 wells of one chamber slide. One Vita-Assay™ RCE AN16 chamber slide can accommodate a total of 1-mL whole blood.

Recommended to use with the nucleated cells prepared by red blood cell lysis of whole blood.

Whole blood, bone marrow or nucleated cells.

Fluorescence microscopy, FISH, Flow cytometry, etc*; Quantitative RT-PCR, DNA microarray, SAGE, proteomics and genomics**.

Vita-Assay™ AN16 series is for the functional enrichment of viable tumor progenitor and other rare stem cells from whole blood, lymph, bone marrow and other bodily fluids. Enriched cells can be directly stained with antibodies and analyzed in the same device by fluorescence microscopy. Enriched cells can also be cultured in the same device for functional analyses of viable tumor progenitor and other rare cells, including immuno-phenotyping and enumeration, as well as CTC drug sensitivity (CDS) testing.

Alternatively:

* CAM-avid cells released from CAM scaffolds by Cell Releasing CAM Enzyme plus Trypsin/EDTA shall be concentrated to a small volume, 100 µL PBS containing the enriched cell fraction. The 100 µL cell suspension can be processed for cellular analyses such as fluorescence microscopy, FISH and flow cytometry.

** CAM-avid cells released from CAM scaffolds by Cell Releasing CAM Enzyme plus Trypsin/EDTA shall be concentrated to a small volume, 10 µL PBS containing the enriched cell fraction. The 10 µL cell suspension can be lysed for protein, mRNA or DNA isolation, followed by proteomics, quantitative RT-PCR, DNA microarray, SAGE and genomic analyses. The sensitivity of qRT-PCR detection can be 1 tumor cell in 1 mL of blood.